Stem Cell Publications


Fasano C.A,, Chambers SM, Lee G, Tomishima MJ, and Studer L. (2010) Efficient derivation of floor plate tissue from human embryonic stem cells.  Cell Stem Cell, 6(4):336-347.

Phoenix TN, Temple S.(2010) Spred1, a negative regulator of Ras-MAPK-ERK, is enriched in CNS germinal zones, dampens NSC proliferation, and maintains ventricular zone structure.
Genes Dev. 1;24(1):45-56.
Fasano, C.A., Studer, L. (2009) Too much Sonic, too little neurons. Nature Neuroscience, 12(2): 1007-8

Fasano C.A, Phoenix TN, Kokovay E, Lowry N, Elkabetz Y, Dimos JT, Lemischka IR, Studer L, Temple S. (2009) Bmi-1 cooperates with Foxg1 to maintain neural stem cell self-renewal in the forebrain. Genes Dev 23:561-74

Chambers, S.M., Fasano, C.A., Papapetrou, E.P., Tomishima, M., Sadelain, M., and Studer, L. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nature Biotechnology, (3) 275-80.

Lee, G, Papapetrou, E. P., Kim, H., Chambers, S.M., Tomishima, M., Fasano, C.A., Viale, A., Tabar, V., Sadelain, M., and Studer, L. (2009) Modeling Pathogenesis and Treatment of Familial Dysautonomia in Patient Specific Pluripotent Stem Cells.  Nature, Sep 17;461(7262):402-6.

Fasano C.A, Phoenix TN, Kokovay E, Lowry N, Elkabetz Y, Dimos JT, Lemischka IR, Studer L, Temple S. (2009) Bmi-1 cooperates with Foxg1 to maintain neural stem cell self-renewal in the forebrain. Genes Dev 23:561-74

Corneo BTemple S. (2009) Sense and serendipity aid RPE generation.Cell Stem Cell. Oct 2;5(4):347-8.

Phoenix TN, Temple S (2009)Baby got brain: Fgf10 sets rostral cortical size..Neuron. Jul 16;63(1):1-3.

Cohen AR, Bjornsson CS, Temple S, Banker G, Roysam B.(2009) Automatic summarization of changes in biological image sequences using algorithmic information theory. IEEE Trans Pattern Anal Mach Intell. Aug;31(8):1386-403.

Okano H, Temple S. (2009) Cell types to order: temporal specification of CNS stem cells Curr Opin Neurobiol.  Apr;19(2):112-9. Epub 2009 May 6. Review

Shen Q, Temple S. (2009) Fine control: microRNA regulation of adult neurogenesis. Nature Neuroscience 12:369-70

Lowry NA, Temple S. (2009) Identifying the perpetrator in medulloblastoma: Dorian Gray versus Benjamin Button. Cancer Cell 15:83-5.

Kokovay E, Shen Q, Temple S. (2008) The incredible elastic brain: how neural stem cells expand our minds. Neuron 60:420-9.

SlaterJL, Landman KA, Hughes BD, Shen Q Temple S. (2009) Cell lineage tree models of neurogenesis. J Theor Biol 256: 164-79.

Shen Q, Wang Y, Kokovay E, Lin G, Chuang SM, Goderie SK, Roysam B, Temple S. (2008) Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions.  Cell Stem Cell 3:289-300.

Lowry N, Goderie SK, Adamo M, Lederman P, Charniga C, Gill J, Silver J, Temple S. (2008) Multipotent embryonic spinal cord stem cells expanded by endothelial factors and Shh/RA promote functional recovery after spinal cord injury. Exp Neurol. 209:510-22.

Kokovay E, Temple S (2007) Taking neural crest stem cells to new heights. Cell 131:234-6

Ashton RS, Peltier J, Fasano CA, O’Neill A, Leonard J, Temple S, Schaffer DV, Kane R. (2007) High-Throughput Screening of Gene Function in Stem Cells using Clonal Microarrays.  Stem Cells. 25:2928-35.

Fasano CA, Dimos JT, Ivanova NB, Lemischka IR, Temple, S (2007) shRNA knockdown of Bmi-1 reveals a critical role for p21/Rb pathway in NSC self-renewal during development. Cell Stem Cell 1:87-99.

De S., Rabin D.; Salero E; Lederman PL; Temple S, Stern, JH (2007) Human RPE cells change to express B-crystallin: A Biomarker for RPE-cell change in Age-Related Macular Degeneration. Archives of Ophthalmology 125: 641-5

Salero E and Hatten ME (2007) Differentiation of ES Cells into Cerebellar Neurons. Proc Natl. Acad. Sci. 20: 104(8):2997-3002.

Lowry NATemple S (2007) Making Human Neurons from Stem Cells after Spinal Cord Injury  PLoS Med. 4:e48

Corneo B, Wendland RL, Deriano L, Cui X, Klein IA, Wong SY, Arnal S, Holub AJ, Weller GR, Pancake BA, Shah S, Brandt VL, Meek K, Roth DB. (2007). Rag mutations reveal robust alternative end joining.  Nature 449(7161):483-6.

Shen Q, Wang J, Dimos JT, Fasano CA, Phoenix TN, Lemischka IR, Ivanova NB,  Stifani S, Morrisey EE, Temple S (2006) The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells. Nature Neuroscience 9:743-51.

Pumiglia K, Temple S.( 2006) PEDF: bridging neurovascular interactions in the stem cell niche. Nature Neuroscience. 9:299-300.

Al-Kofahi O, Radke RJ, Goderie SK, Shen Q, Temple S, Roysam B. (2006) Automated cell lineage construction: a rapid method to analyze clonal development established with murine neural progenitor cells. Cell Cycle  5:327-35.

Stern JH, Temple, S, De S.  (2006) Retinal Repair by Stem Cell Transplantation.  Chapter in: Stem Cell and Gene Based Therapy: Frontiers in Regenerative Medicine, editors Battler A. and Leor J, Springer-Verlag, pp. 259-80.

Capela A, Temple S. (2006) LeX carbohydrate expression by progenitor cells with high growth and neurogenic potential in the embryonic nervous system. Developmental Biology  291:300-13.

Natalia Abramova, Carol Charniga, Susan K. Goderie, Sally Temple (2005) Stage-specific changes in gene expression in acutely isolated mouse CNS progenitor cells. Developmental Biology 283:269-281.

Yu Sun, Goderie Susan K., Temple S. (2005) Asymmetric distribution of EGF receptor during mitosis generates diverse CNS progenitor cells. Neuron 45:873-886.

Shen, Q., Goderie, S., Jin, L., Karanth, N., Sun, Y., Abramova, N., Vincent, P., Pumiglia,K., Temple, S. (2004) Endothelial Cells Stimulate Self-Renewal and Expand Neurogenesis of Neural Stem Cells. Science 304:1338-1340

Li HS, Wang D, Shen Q, Schonemann MD, Gorski JA, Jones KR, Temple S, Jan LY,
Jan YN. (2003). Inactivation of Numb and Numblike in embryonic dorsal forebrain impairs neurogenesis and disrupts cortical morphogenesis. Neuron 40:1105-18.

Temple S. (2003) Embryonic stem cell self-renewal, analyzed. Cell 115:247-8.

Salero E, Gimenez C, Zafra F (2003) Identification of a non-canonical E-box motif as a regulatory element in the proximal promoter region of apolipoprotein E gene. Biochem J 370: 979-86.

Shen Q, Temple S.  (2002). Creating asymmetric cell divisions by skewing endocytosis.  Review Science STKE 162:PE52.

Schneider AS, Atluri P, Shen Q, Barnes W, Mah SJ, Stadfelt D, Goderie SK, Temple S, Fleck MW. (2002)  Functional nicotinic acetylcholine receptor expression on stem and progenitor cells of the early embryonic nervous system. Ann N Y Acad Sci. 971:135-8.

Capela A, Temple S. (2002). LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal. Neuron 35:865-75.

Shen Q, Zhong W, Jan YN, Temple S. (2002).  Asymmetric Numb distribution is critical for asymmetric cell division of mouse cerebral cortical stem cells and neuroblasts. Development 129:4843-53.

Corneo B, Benmerah A, Villartay JP. (2002). A short peptide at the C terminus is responsible for the nuclear localization of RAG2. Eur J Immunol. 32:2068-73.

Temple S. (2001) The development of neural stem cells. Nature 414: 112-118.

He W., Ingraham C., Rising L., Goderie S., Temple S. (2001) Multipotent stem cells from the mouse basal forebrain contribute GABAergic neurons and oligodendrocytes to the cerebral cortex during embryogenesis. J. Neurosci. 21: 8854-62

Atluri P, Fleck MW, Shen Q, Mah SJ, Stadfelt D, Barnes W, Goderie SK, Temple S, Schneider AS (2001). Functional nicotinic acetylcholine receptor expression in stem and progenitor cells of the early embryonic mouse cerebral cortex. Dev Biol. 240:143-56.

Temple S. (2001) Stem cell plasticity—Building the brain of our dreams. Nat Rev Neurosci. 2:513-20.

Temple, S.  (2001) Defining neural stem cells and their role in normal development of the nervous system.  Chapter in: Neural stem cells, ed. M. Rao, Humana Press.

Salero E, Perez Sen R, Aruga J, Gimenez C and Zafra F (2001) Transcription factors Zic1 and Zic2 bind and transactive the apolipoprotein E gene promoter. J Biol Chem 276: 3 18881-8.

Yan J, Studer L, McKay RD. J  (2001) Ascorbic acid increases the yield of dopaminergic neurons derived from basic fibroblast growth factor expanded mesencephalic precursors. Neurochem. Jan;76(1):307-11.

Moshous D., Callebaut I., de Chasseval R., Corneo B., Cavazzana-Calvo M., Le Deist F., Tezcan I., Sanal O., Bertrand Y., Philippe N., Fischer A. and de Villartay J.P. (2001) Artemis, a novel DNA double strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency.  Cell 105: (177-186).

Corneo B., Moshous D., Güngor T., Wulffraat  N., Philippet  P., Le Deist F., Fischer  A., and de Villartay J.P. (2001) Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-SCID or Omenn syndrome. Blood  97 (9) : 2772-2776.

Qian, X., Shen, Q., Goderie, S.K., He, Wenlei, Capela, A. and Temple, S. (2000) Timing of CNS cell generation: a programmed sequence of neron and glial cell production from islated murine cortical stem cells Neuron 28: 69-80.

Corneo B., Moshous D., Callebaut I., de Chasseval R., Fischer A., de Villartay J.P.  (2000) Three-dimensional clustering of human Rag2 gene mutations in severe combined immunodeficiency. J. Biol . Chem. 275 (17) :12672-12675.

Temple, S. (1999) CNS development: The obscure origins of the adult stem cells.  Review, Current Biol., 9:R397-9.

Temple, S. and Alvarez- Buylla, A. (1999) Stem cells in the adult mammalian central nervous system. Review, Curr. Opin. Neurobiol. 9:135-41.

Shen, Q., Qian, X., Capela, A. and Temple, S (1998). Stem cells in the embryonic cerebral cortex – their role in histogenesis and patterning.  Review, J. Neurobiol. 36: 162-174.

Yan J and  Barrett JN Purification from bovine serum of a survival-promoting factor for cultured central neurons and its identification as selenoprotein P. J Neurosci. 1998 Nov 1;18(21):8682-91.

Alvarez-Buylla, A. and Temple, S. (1998) Stem cells in the developing and adult nervous system.  J. Neurobiol. 36: 105-110.

Qian, X., Goderie, S.K., Shen, Q., Stern, J.H. and Temple, S. (1998) Intrinsic Programs of Patterned Cell Lineages in Isolated Vertebrate CNS Ventricular Zone Cells, Development 125: 3143-3152.

Qian, X., Davis A.A., Goderie, S.K. and Temple, S. (1997).  FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells.   Neuron 18: 81-93.

Strazzabosco M., Corneo B., Iemmolo R.M., Menin C., Gerunda G., Bonaldi L., Merenda R., Neri D., Poletti A., Montagna M., Del Mistro A., Maffei Faccioli A., D’Andrea E. (1997) Epstein-Barr virus-associated post-transplant lympho-proliferative disease of donor origin in liver transplant recipients. J. Hepatol. 26 (4) : 926-934.

Temple, S. (1996) Out of the shadows.  Book review, Nature 381.

Temple, S. and Qian, X. (1996) Vertebrate neural progenitor cells: sub-types and regulation Curr. Opinion in Neurobiol.  6: 11-17.

Lah TT, Calaf G, Kalman E, Shinde BG, Somer R, Estrada S, Salero E, Russo J and Daskal I (1996) Capthepsins D, B and L in transformed human breast epithelial cells. Breast Cancer Res Treat 39: 2 221-233.

Montagna M., Santacatterina M., Corneo B., Menin C., Serova O., Lenoir G.M., Chieco-Bianchi L., D’Andrea E. (1996) Identification of seven new BRCA1 germline mutations in Italian breast and breast-ovarian cancer families. Cancer Res., 56 (23) : 5466-5469.

Menin C., Indraccolo S., Montagna M., Corneo B., Bonaldi L., Leib-Mosch C., Chieco-Bianchi L., D’Andrea E. (1996). Identification of a human endogenous LTR-like sequence using HIV-I LTR specific primers. Molec. Cell.Probes, 10 (6) : 443-451.

Temple, S. and Qian, X.  (1995)  bFGF, Neurotrophins and the Control of cortical Neurogenesis. Neuron 15: 249-252.

Salero E, Vergara, P and Segovia, J (1995) Intracellular increases of cAMP induce opposite effects in glutamic acid descarboxilase (GAD67) and glial fibrillary acidic protein immunoreactivities in C6 cells. Neurosci Lett 191: 1-2 9-12.

Menin C., Ometto L., Veronesi A., Coppola V., Veronese M. L., Indraccolo S., Bruni L., Corneo B., Amadori A., De Rossi A., Chieco-Bianchi L., D’Andrea E. (1995). Dominance of a single Epstein-Barr virus strain in SCID mouse tumors induced by injection of peripheral blood mononuclear cells from healthy human donors. Virus Res., 36 (2-3): 215-231.

Davis, A.A. and Temple, S. (1994) A self-renewing, multipotential stem cell in embryonic rat cerebral cortex.   Nature 372: 263-266.

Temple, S. and Davis, A.A. (1994)   Isolated rat cortical progenitor cells are maintained in division in vitro by membrane-associated factors.  Development 120:999.

Nonner, D, Temple S. and Barrett J.N. (1992) Rat embryonic septal neurons survive and express cholinergic properties in isolation and without nerve growth factor.  Develop Brain Res. 70,197-205.

Temple, S. (1990) Characteristics of cells that give rise to the central nervous system.  Review, J. of Cell Science 97: 213-219.

Temple S. (1989) Division and differentiation of isolated CNS blast cells in microculture.  Nature 340:471-473.

Raff, M.C., Temple,S. and ffrench-Constant,C. (1987)  Glial cell development and function in the rat optic nerve. Prog. In Brain Res. 71:435-438.

Temple, S. and Raff, M.C. (1986) Clonal analysis of oligodendrocyte development in culture: Evidence for a developmental clock that counts cell divisions. Cell 44:773-779.

Temple, S. (1986) Single cell studies of optic nerve development. PhD dissertation, University College London, England.

Temple, S. and Raff, M.C. (1985) Differentiation of a bipotential progenitor cell in single cell microculture.  Nature 313:223-225.